The Moli-sani Study is one of the largest population cohorts in Europe. Established in 2005-2010 with the purpose of investigating genetic and environmental risk factors in the onset of main chronic-degenerative diseases, the Study has recruited 24,325 men and women aged ≥35 years residing in the southern Italian region of Molise.
At baseline, participants underwent an in-depth medical examination, including electrocardiogram and spirometry, as well as a thorough assessment of diet, general health, and lifestyle.
The Moli-sani Study cohort is followed up every three years for mortality and incidence of chronic diseases through record linkage to regional death register and hospital discharge form registers. The validation of major events is achieved by checking hospital medical records using international standardized criteria.
Along with the start of the study, a population Biological Bank has been established to store participants’ biological material (plasma citrated, plasma EDTA, serum, buffy-coat and urine) in liquid nitrogen (-196 °C).